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. 2012 Dec 6;7(12):e50754. doi: 10.1371/journal.pone.0050754

Figure 2. Erythrocyte binding activity of 15 different P. vivax tryptophan rich antigens (PvTRAgs) by Flowcytometry.

Figure 2

Total erythrocytes were used for the binding assay. Dot plot and Histogram of erythrocytes gates were used for the analysis. Bar diagram shows the binding of all different PvTRAgs with the erythrocytes or reticulocytes (inset). One million RBC were incubated with 1 µM concentration of Histidine-tagged recombinant proteins. Histidine-tagged PvATRAg74 and bacterial recombinant thioredoxin were used as positive and negative controls, respectively. Data shown are the mean ± S.D. of at least 3 independent experiments. The difference between PvTRAgs and thioredoxin binding to erythrocytes was significant (p<0.001). Background, no PvTRAg was added.