Figure 3. Competitive inhibition of PvTRAgs binding to erythrocytes by.

Cell ELISA. A mixture of Histidine-tagged recombinant PvTRAg (200 nM) and variable amount (0.0 to 2 µM) of respective untagged PvTRAg was added to ∼1 million RBCs. After incubation, the plates were developed by the anti-histidine antibodies as in Fig.1 legend. a, PvTRAg35.2; b, PvTRAg69.4; c, PvTRAg38; d, PvTRAg33.5; e, PvATRAg74; f, PvTRAg. Data shown are the mean ± S.D. of at least 3 independent experiments.