Figure 4. Competitive inhibition of PvTRAgs binding to erythrocytes by Flowcytometry.

Equimolar concentration of respective Histidine-tagged and untagged PvTRAg proteins (1 µM each) were added to the (∼1 million) erythrocytes. Competition was not allowed for normal binding, it was used as control. Thioredoxin was used as negative control. Data shown are the mean ± S.D. of at least 3 independent experiments. Background, no PvTRAg was added.