Skip to main content
. 2012 Dec 6;7(12):e50754. doi: 10.1371/journal.pone.0050754

Figure 5. Specificity of PvTRAgs binding to erythrocytes by Cell – ELISA.

Figure 5

ELISA plate was coated with one million RBC and incubated with 1 µM of untagged PvTRAgs (A) or untagged thioredoxin (B) followed by incubation with different concentrations of Histidine-tagged recombinant proteins. The plate was developed with anti-histidine antibodies as in Fig.1. In the negative control well, Histidine-tagged thioredoxine was added at different concentrations. For Inset, the ELISA plate coated with one million erythrocytes was incubated with untagged PvTRAgs followed by incubation with Histidine-tagged thioredoxin at different concentrations. Plates were developed as in above. Data shown are the mean ± S.D. of at least 3 independent experiments.