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. 2012 Dec 6;7(12):e50754. doi: 10.1371/journal.pone.0050754

Figure 6. Inhibition of erythrocyte binding activity by patients’ sera.

Figure 6

Histidine-tagged PvTRAgs (250 nM) were mixed with different dilutions of P.vivax patients’ sera before adding to the microtiter plate coated with one million erythrocytes. Further steps of colour development were same as in Fig.1. a, PvTRAg35.2; b, PvTRAg69.4; c, PvTRAg38; d, PvTRAg33.5; e, PvATRAg74; f, PvTRAg. Data shown are the mean ± S.D. of at least 3 independent experiments. No Ab, no antibody but only 1×PBS; HCS, healthy control sera from humans.