Figure 8. Effect of enzyme treatment of erythrocytes on their binding capacity to PvTRAgs.

Human erythrocytes were pretreated with Trypsin, Chymotrypsin or Neuraminidase prior to their coating to the microtiter plate. Then Histidine-tagged recombinant PvTRAgs (1 µM) or P.vivax duffy binding protein region II (PvRII) were used for the binding assay with these treated RBCs using the same steps as in Fig1. Untreated RBCs were taken as controls. Data shown are the mean ± S.D. of at least 3 independent experiments.