Figure 9. Sharing of RBC receptors by different PvTRAgs.

Microtiter plate coated with ∼1 million erythrocytes was incubated with 1 µM of untagged PvTRAg (A), PvTRAg69.4 (B), PvTRAg35.2 (C), PvTRAg33.5 (D), PvTRAg38 (E), PvATRAg74(F), or PvTRAg38 plus PvATRAg74 (G). After washing with PBS, 1 µM of Histidine tagged PvTRAgs were added and binding was detected by monoclonal anti-His₆ antibody as described in text. Mean ± SD value of percent binding of three different experiments is shown. Binding of PvTRAgs without competitor was considered as 100%.