Table 2. Cell-ELISA based cross-inhibition of PvTRAgs binding to erythrocytes by rabbit antibodies raised against the same antigen or against its close homolog.
| Antigens | Erythrocyte Binding Inhibition* | |
| Self Antibody%±S.D. | Homolog Antibody %±S.D | |
| PvTRAg38 | 52.4±2.3 | 31±1.6 (PvATRAg74) |
| PvATRAg74 | 64.8±0.8 | 43.6±2.2 (PvTRAg38) |
| PvTRAg33.5 | 64.5±0.7 | 41.8±6.9 (PvTRAg35.2) |
| PvTRAg35.2 | 66.8±0.7 | 42.6±1.0 (PvTRAg33.5) |
| PvTRAg | 52.9±2.6 | 34.2±0.6 (PvTRAg69.4) |
| PvTRAg69.4 | 63.5±0.3 | 42.9±5.6 (PvTRAg) |
*
In a cell-ELISA assay, the Histidine tagged PvTRAgs were incubated with polyclonal antisera raised in rabbits at 1∶100 dilutions against the same antigen or its closest homolog (shown in brackets). Pre-incubated mixture was then allowed to react with erythrocytes and binding was detected by monoclonal anti-His6 antibody as described in the text. Binding of PvTRAgs in the absence of antibody is taken as100%. Values are mean ± standard deviation of three different experiments. The erythrocyte binding inhibition between self antibody and homologue antibody was significantly different (P<0.05).