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. 2012 Dec 6;7(12):e50928. doi: 10.1371/journal.pone.0050928

Figure 3. Follicular proliferation.

Figure 3

Immunohistochemical localization of PCNA in ovarian follicles was studied 24-h after irradiation. (A) Expression of PCNA in ovaries of the control group. (B) Expression of PCNA in ovaries treated with sodium selenite (0.5 mg/kg) alone. (C) Expression of PCNA in ovaries exposed to γ-radiation (3.2 Gy). (D) Expression of PCNA in ovaries treated with sodium selenite (0.5 mg/kg) for one week before being exposed to γ-radiation (3.2 Gy). Scale bar, 10 µm. (E) Quantitative image analysis for IHC staining expressed as a percentage of PCNA positive cells against the total number of granulosa cells across seven higher power fields (40×) for each rat section. Each column represents the mean ± SD of at least three independent experiments. a or b: Statistically significant from control or irradiated group, respectively at P<0.05 using one-way ANOVA followed by Tukey–Kramer as a post-hoc test.