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. 2012 Dec 6;7(12):e50928. doi: 10.1371/journal.pone.0050928

Figure 7. Immunohistochemical localization of caspase 3.

Figure 7

(A & B) Expression of caspase 3 in ovaries and uterus of control groups shows a minimal degree. (C & D) Expression of caspase 3 in ovaries and uterus treated with sodium selenite (0.5 mg/kg) alone shows a minimal degree. (E & F) Expression of caspase 3 in ovaries and uterus exposed to γ-radiation (3.2 Gy) shows an extensive degree. (G & H) Expression of caspase 3 in ovaries and uterus treated with sodium selenite (0.5 mg/kg) for one week before being exposed to γ-radiation (3.2 Gy) was limited. (I & J): Ovarian and uterine negative controls omitting primary antibody. Scale bar, 10 µm. (K): Quantification of ovarian caspase 3 staining represents the percent of immunopositive cells to the total area of the microscopic field (20×); averaged across 7 fields for each rat section. Each column represents the mean ± SD of at least three independent experiments. a or b: Statistically significant from control or irradiated group, respectively at P<0.05 using one-way ANOVA followed by Tukey–Kramer as a post-hoc test.