Figure 2. Functional complementation.

Relative primary root lengths are shown for NIL[Sha] plants and for transgenic NIL[Sha] plants expressing either the FRD3^Bay or the FRD3^Sha allele. Relative primary root length is the primary root length of plants grown at 150 µM Zn as a percentage of the primary root length of plants grown at 1 µM Zn. Error bars represent confidence intervals calculated after logarithmic transformation of data [41] (P<0.05; n = 7 to 21). Similar increased relative root lengths were observed in 2 additional independent NIL[Sha] lines complemented with FRD3^Bay (data not shown).