Figure 2. Interpretation of the results of MAPS.
(A). A representative mapping of the paired reads from MAPS at integration sites. Read 1 sequences are anchored, since they extend from the fixed HBX nested primer; while the positions of Read 2 sequences vary due to the random fragmentation. (B). Insertion sites illustration in UCSC genome browser. (i) Insertion in ERBB4. (ii) Insertion in GRM8. (C). Integrant sequences of ERBB4 and GRM8. Breakpoint is indicated by arrow. The numbers are HBV genome coordinates, based on the reference of GQ205441. The human gene sequences are underlined. Orange bases indicate the common base between HBV and Human at the junction. (D). Distribution of integrant lengths in the sequenced library of ERBB4 and GRM8 integrations.