Table 1. Summary of immunofluorescence-based colocalization studies of HCV proteins and cellular marker proteins.
| cellular proteins | viral proteins | ||||||
| organelle | marker | core | E2 | NS3 | NS4B | NS5A | |
| dsRNA | 0.41 | 0.3 | 0.34 | 0.23 | 0.36 | ||
| rough ER | PDI | 0.58 | 0.67 | 0.63 | 0.53 | 0.52 | |
| GRP94 | 0.72 | 0.58 | 0.74 | 0.54 | 0.43 | ||
| smooth ER | EH | 0.34 | n.a. | 0.43 | n.a. | 0.35 | |
| reticular subdomain of rough ER | CLIMP-63 | 0.37 | 0.39 | 0.59 | 0.323 | 0.4 | |
| LD | BODIPY | 0.6 | 0.36 | 0.32 | 0.54 | 0.37 | |
| ADRP | 0.82 | 0.42 | 0.42 | 0.44 | 0.56 | ||
| mitochondria | MitoTracker | 0.48 | 0.53 | 0.6 | 0.62 | 0.5 | |
| ER-Golgi intermediate compartment | GFP-ERGIC-53 | 0.3 | 0.31 | 0.31 | n.a. | 0.34 | |
| cis Golgi network | GOS-28 | 0.29 | 0.53 | 0.34 | 0.24 | 0.4 | |
| trans Golgi network | TGN46 | 0.27 | n.a. | 0.23 | n.a. | 0.24 | |
| early endosome | GFP-Rab21 | 0.6 | 0.57 | 0.75 | n.a. | 0.71 | |
| Rab5 | 0.27 | n.a. | 0.26 | n.a. | 0.30 | ||
| EEAI | 0.2 | n.a. | 0.16 | n.a. | 0.28 | ||
| autophagosome | GFP-LC3 | 0.21 | 0.21 | 0.14 | n.a. | 0.03 | |
| vesicular trafficking | COP I | YFP-β COP | 0.5 | 0.48 | 0.44 | n.a. | 0.52 |
| COP II | Sec13 | 0.43 | n.a. | 0.34 | n.a. | 0.35 | |
| recycling compartment | Rab11 | 0.3 | n.a. | 0.39 | n.a. | 0.36 | |
| GFP-Rab11 | 0.33 | 0.63 | 0.28 | n.a. | 0.51 | ||
| late endosomes | Lamp-3 | 0.32 | 0.17 | 0.19 | 0.19 | 0.25 | |
| GFP-Rab7A | 0.75 | 0.7 | 0.54 | n.a. | 0.78 | ||
| multivesicular bodies | LBPA | 0.40 | 0.30 | 0.36 | 0.3 | 0.3 | |
Naïve high-passage Huh-7 cells were infected with 30 TCID50/cell of Jc1 and 48 h later processed for indirect immunofluorescence. Signal intensities were quantified by using Intensity Correlation Analysis (ICA) of the Image J software package and are given as Pearson's correlation coefficient (PCC). PCC can range from −1 to 1, corresponding to no or perfect colocalization. Only values higher than 0.5 were considered as colocalization.
n.a., not applicable owing to incompatible antibodies.
ADRP, adipose differentiation-related proteins or adipophilin; CLIMP-63, cytoskeleton linking membrane protein 63; COP I, coat protein I; COP II, coat protein II; EEA1, early endosome antigen 1; EH, epoxide hydrolase; ERGIC-53, ER Golgi intermediate compartment; GOS-28, Golgi SNARE protein 28; GRP94, glucose related protein 94; Lamp-3, lysosome-associated membrane protein-3; LBPA, lysobisphosphatidic acid; LC-3, light chain protein 3; PDI, protein disulphide isomerase; Rab5, Ras-related GTP binding protein 5; Rab7A, Ras-related GTP binding protein 7A; Rab11, Ras-related GTP binding protein 11; Rab21, Ras-related GTP binding protein 21;. sec13, secretory protein 13; TGN46, Trans Golgi network protein 46.