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RNA gel-blot hybridization analysis of PNZIP mRNA levels during an extended dark treatment. Seedlings were grown in continuous light for 6 d and than transferred to darkness for 0 to 48 h. At the hours indicated, plants were harvested and their RNA was isolated. Each lane contained 30 μg of cotyledon total RNA. Hybridization to a ubiquitin cDNA probe served as a control for equal loading of RNA in each lane. Numbers on the right indicate the approximate sizes of the mRNAs detected.
