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. 1998 Jan;116(1):27–35. doi: 10.1104/pp.116.1.27

Figure 4.

Figure 4

Effects of end-of-day treatments of P. nil seedlings with red and far-red light on flowering and PNZIP mRNA accumulation during the dark. Seedlings were grown in continuous light for 6 d, and just before the transition to darkness at 0 h they were exposed for 10 min to red (R), far-red (FR), far-red and then red (FR/R), or red and afterward far-red (R/FR) light. A, Effects of end-of-day treatments on flower induction as measured after 3 weeks. Error bars indicate ses. B, RNA gel-blot hybridization analysis of PNZIP mRNA levels detected 4 and 14 h after the end-of-day treatments. Each lane contained 30 μg of cotyledon total RNA. A photograph of the amount of rRNA served as a control for equal loading of RNA in each lane. Numbers on the right indicate the approximate sizes of the mRNAs detected. The black and white bar at the bottom of the figure schematically presents the light/dark conditions and the timings of light treatments and RNA isolation.