Figure 5.

RNA gel-blot hybridization analysis of PNZIP mRNA accumulation during continuous darkness or following an NB treatment. Seedlings were grown in continuous light for 6 d and then treated with three durations of darkness (DK 12, 16, or 20 h) or were interrupted at the 8th h of dark treatment by a 10-min NB with red light. Each lane contained 30 μg of cotyledon total RNA. Hybridization to a ubiquitin cDNA probe served as a control for equal loading of RNA in each lane. Numbers on the right indicate the approximate sizes of the mRNAs detected. The black and white bars at the bottom of the figure schematically presents the light/dark conditions and the timings of the NB treatment and RNA isolation.