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Lipase clones from metagenome of activated biomass. Lipase activity was identified by disappearance of blue colour from tributyrin plates (containing spirit blue as an indicator). Panel a shows the control plate where overnight grown E. coli DH5α containing pUC18 (1) and E. coli DH10B containing pBeloBAC11 (2) have been spotted. Panel b and c show independent positive clones. (Color figure online)
