Table 1.

Characteristics of metagenomic libraries developed from activated biomass of an ETP treating wastewater generated at a pharmaceutical industry

Vector used in library construction	Total no. of clones in library	Average insert size1	Representative bacterial metagenome screeneda,b	Activity in clone	No. of clones
pBeloBAC11	40,000	11 kb	95.6 times of bacterial genome	Lipase dioxygenasec	12 4
pUC18	32,000	6 kb	41.7 times of bacterial genome	Cellulase	4

^aBased on the size of E. coli genome (4.6 Mb)

^bMultiplied total number of clones by average insert size, viz., 11.0 kb for pBeloBAC11 library and 6.0 kb for pUC18 library

^cBiotransformation of catechol based on plate assay [30]

¹Calculations of average insert size in (supplementary Figs. 1 and 2)