SDS-PAGE of different protein samples. Lane 1 xynZF-2 purified by Ni2+-chelating chromatography, lane 2E. coli pET-28a-xynZF-2 with IPTG induction, lane 3E. coli pET-28a-xynZF-2, lane 4E. coli pET-28a(+) with IPTG induction, lane 5E. coli pET-28a(+), lane M protein markers with the following molecular weight standards: 12, 20, 30, 40, 60, 80 kDa