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. 2012 Nov 14;189(12):5755–5763. doi: 10.4049/jimmunol.1200804

FIGURE 5.

FIGURE 5.

3D8-OVA250–264 is cross presented via the classical cytosolic MHC class I pathway. DC2.4 cells were pulsed with 3D8-OVA250–264 (5 μM) or OVA257–264 peptide (1 μM) in the presence or absence of the indicated inhibitors for 6 h at 37°C. DC2.4 cells were washed, fixed, and then incubated with CD8OVA1.3 T hybridoma cells for 24 h at 37°C. IL-2 production is expressed as a ratio against the amount of IL-2 in the absence of each inhibitor. Data represent the mean ± SE from four independent experiments. *p < 0.005, Student t test.