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In vivo cytotoxic T cell stimulation of 3D8-OVA250–264. Splenocytes from C57BL/6 mice immunized with the indicated proteins (50 μg) or PBS were subjected to a 51Cr release assay using B16 (A) or MO5 (B) as target cells. Data represent the mean ± SE of two independent experiments performed in triplicate. *p < 0.005, Student t test.
