Figure 1.

Determination of tumour clonality by HUMARA. DNA isolated from primary tumours, secondary tumours, and non-neoplastic lymph-node tissues from each patient were incubated with (+) methylation-specific HpaII and were amplified with primers for exon 1 of AR. As a negative control, samples were incubated without methylation-specific HpaII under the same conditions. A methylated AR gene was found on different alleles in cases 7 and 8 (in ipsilateral tumour samples) and cases 14–19 (in contralateral tumour samples).