Fig. 1.

Immunohistological analysis of human atherosclerotic lesions. HSP60 expression (red) was determined in (A) ECs at arterial bifurcation areas stressed by turbulent blood flow conditions (vWF⁺, green, 200×, scale bar 200 μm) with simultaneous expression of adhesion molecules as (B) VCAM-1 (CD106, green, scale bar 200 μm), (C) CD106, and (D) CD62E (40×, scale bar 100 μm). Mononuclear cells in the intima expressed (E) CD40⁺ (green) and (F) HLA-DR⁺ (green, 200×, scale bar 200 μm). Subsets of (G) CD3⁺, (H) macrophages (CD68⁺, brown), (I) mast-cells (tryptase⁺), (J) DCs (CD1a⁺), (K) CD4⁺ T-cells, and (L) CD8⁺ T-cells were identified infiltrating the intima. (M) CD3⁺, (N) CD4⁺, (O) CD8⁺ T-cells, and (P) CD68⁺ were identified in advanced atherosclerotic lesions. Images were acquired using a Nikon Eclipse E800 microscope. Original magnification 600× (scale bar 100 μm). All immunohistological stainings and analysis of these were performed at least three times. A representative immunohistological analysis from 4 EL and 8 LL donors are demonstrated here.