TABLE 2.

Mass and oligomeric state of hRap1, hTRF2, and the TRF2-Rap1 complex on telomeric Holliday junctions

Mass, mean area, and the oligomeric state of each protein were obtained from the two-dimensional projection analysis of the tungsten shadowcast images as in Figs. 1 and 4. Masses were calculated based on using ferritin as a size standard. Area distributions with Gaussian fits are shown in supplemental Fig. 5.

Bound to telomeric Holliday junction	No. of molecules counted	Mean area	Calculated mass	95% Confidence Interval +/−	No. of oligomers
		pixel	kDa	kDa
TRF2	168	13,771	315	1.6	Tetramer
Rap1	87	10,472	256	2.1	Tetramer
TRF2-Rap1 complex	119	15,823	475	3.3	Tetramer of TRF2 and Rap1