TABLE 3.
Affinity of hRap1, hTRF2, and the TRF2-Rap1 complex on telomeric and nontelomeric DNA templates
The dissociation constants of hRap1, hTRF2, and the TRF2-Rap1 complex on the telomeric and nontelomeric DNA templates with a 3′ overhang or on the duplex telomere DNA are presented derived from electrophoretic experiments shown in the supplemental Figs. 6 and 7. The Hill coefficient values of each protein on the model telomeric DNA are shown with the error values.
| Hill coefficient |
KD |
|||
|---|---|---|---|---|
| Telomeric 3′ overhang | Duplex telomere template | Nontelomeric 3′ overhang | ||
| nm | nm | nm | ||
| hRap1 | 2.4 ± 0.4 | 118.9 ± 12.1 | 265.0 ± 57.2 | 161.3 ± 25.3 |
| hTRF2 | 5.3 ± 1.3 | 64.7 ± 3.4 | 85.5 ± 11.6 | 133.0 ± 16.4 |
| hTRF2-hRap1 | 3.3 ± 0.6 | 5.8 ± 0.3 | 37.0 ± 9.1 | No binding |