FIGURE 5.

NQO1 with terminal deletions fails to interact with C/EBPα. A, schematic showing of C- and N-terminal deletions of NQO1 (left panel). V5-tagged NQO1 and deletion mutants were transfected and immunoblotted to demonstrate input of expressed proteins (right panel). B, V5- tagged NQO1 deletion mutants were co-transfected with Flag-C/EBPα. The transfected cells were lysed and immunoprecipitated with IgG (control), anti-Flag and anti-V5. Immunoprecipitated proteins were separated on SDS-PAGE and immunoblotted. The immunoblots were probed with anti-V5 to detect NQO1 deletions, anti-Flag to detect Flag-C/EBPα and anti-20S 5α to detect 20S. All input were 10% of the proteins used in the experiments.