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. 2012 Oct 12;287(50):41667–41683. doi: 10.1074/jbc.M112.390781

FIGURE 1.

FIGURE 1.

Expression of GIV mRNA and protein is up-regulated during epithelial wound healing and cancer progression. A, confluent HeLa cell monolayers were scratch-wounded and harvested at the indicated time points after wounding, and whole cell lysates were analyzed for full-length GIV mRNA (left) and protein (right) by qPCR and immunoblotting (IB), respectively. Changes in GIV mRNA are expressed as mean ± S.D. (n = 3 separate experiments). B, -Fold changes in GIV mRNA observed immediately after an acute surgical wound of human skin and at 3 and 7 days (d) after wounding were analyzed using the raw microarray data reported in a study by Nuutila et al. (30) investigating the human skin transcriptome during wound healing. All values were normalized to intact normal skin. C, the abundance of GIV mRNA in highly metastatic MDA-MB 231 breast cancer cells (left) and DLD-1 colorectal cancer cells (right) was analyzed by qPCR, normalized to their respective normal controls, and expressed as -fold change in GIV mRNA. D, the abundance of GIV mRNA was analyzed in the isogenic background of the 21T series of breast cancer cells (left) and Ls series of colorectal cancer cells (right) by qPCR, normalized to the least invasive cell line in each series (i.e. 16N in breast and Ls-174T in colon), and expressed as -fold change in GIV mRNA. Error bars represent S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001.