FIGURE 5.

BDNF stimuli retards LIMK1 degradation through TrkB/LIMK1 interaction. A, BDNF stimuli increased LIMK1 protein levels. Cultured hippocampal neurons were serum-starved and treated with vehicle or 50 ng/ml BDNF for the indicated times, and then cells were lysed and Western blot analysis was performed. Con, control. B, quantification of LIMK1 levels (n = 3, *, p < 0.05 versus 0 h; Student's t test). C, retarded LIMK1 degradation by BDNF treatment can be blocked by Tat-JMBox4 peptide. Serum-starved hippocampal neurons were treated with CHX (20 μg/ml) and/or Tat-JMBox4 (1 μm) 30 min before BDNF (50 ng/ml) administration. After the indicated times, neurons were lysed for Western blot analysis. D, quantification of LIMK1 levels (n = 3, *, p < 0.05; **, p < 0.01 versus 0 h; one-way ANOVA).