FIGURE 2.

Altered starch content in mutant plants lacking combinations of AMY3, ISA3, and LDA. Plants were grown for 28 days (A) or 35 days (B) in a 12-h light/12-h dark regime and harvested at the indicated time points. Samples comprising all of the leaves from a single plant were extracted using perchloric acid. Starch in the insoluble fraction was measured after enzymatic hydrolysis to glucose. Each point is the mean ± S.E. (error bars) (n = 4). A, Starch in wild-type (black diamonds), amy3 (red squares), lda (green triangles), and amy3lda plants (blue circles). B, starch in isa3 (blue diamonds), isa3lda (red squares), amy3isa3 (green triangles), and amy3isa3lda plants (purple circles). All genotypes have elevated starch compared with the wild type (A).