FIGURE 5.

Diurnal changes of branched glucans in the isa3lda double mutant. Four replicate plants were harvested at each time point indicated during the diurnal cycle and immediately frozen in liquid N₂. Malto-oligosaccharides were analyzed by HPAEC-PAD. Representative chromatograms are shown. A, malto-oligosaccharides in isa3lda extracts during the day. EN, end of night. Time in the light is indicated. Elution times of glucans with d.p. 6–8 are indicated by dashed lines. Note the differences in relative abundance of several chain species at the beginning of the day (e.g. glucan species eluting between d.p. 7 and 8). B, malto-oligosaccharides present in isa3lda extracts during the night. ED, end of day. Time in the dark is indicated. Note that there are no differences in relative abundance of the chain species.