TABLE 2.

Starch content during an extended night period

Plants were grown for 35 days in a 12-h light/12-h dark regime. After a normal night, plants were subjected to a night phase extended by 48 h. Samples comprising all leaves from a single plant were harvested at time points during the extended night and extracted using perchloric acid. Starch in the insoluble fraction was measured after enzymatic hydrolysis to glucose. Each value is the mean ± S.E. (n = 4). Footnotes indicate values that differ significantly from the normal end-of-night values of the corresponding genotype (Student's t test). NA, not analyzed.

Genotype	Normal night (12-h dark) starch	Extended night starch
		36-h dark	60-h dark
	mg/g FW	mg/g FW	mg/g FW
Wild type	0.25 ± 0.08	NA	NA
isa3lda	19.76 ± 0.27	12.52 ± 0.85a	10.13 ± 1.61a
amy3isa3	37.52 ± 2.97	25.34 ± 2.29b	24.87 ± 1.22a
amy3isa3lda	46.08 ± 5.01	47.47 ± 3.10	45.09 ± 3.13
gwd1	50.39 ± 4.30	40.30 ± 0.33b	36.99 ± 3.40b

^a p ≤ 0.01.

^b p ≤ 0.1.