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. 2012 Oct 16;287(50):41875–41887. doi: 10.1074/jbc.M112.421552

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — PEPCK and Glc-6-Pase play a significant role in cAMP-induced steroidogenesis. A and B, MA-10 cells were transfected with shPEPCK (200 ng) or treated with 3-MPA (10 μm) and cultured in the presence of cAMP (100 μm) for the indicated time period, and media were collected for progesterone (left panel) and testosterone (right panel) measurement by RIA. Each point represents the average concentration of progesterone and testosterone per cell from three independent experiments. Data are representative of two independently conducted experiments. C and D, MA-10 cells were transfected with siGlc-6-Pase (100 pmol) or treated with S 3483 (10 μm) and cultured in the presence of cAMP (100 μm) for the indicated time period, and media were collected for progesterone (left panel) and testosterone (right panel) measurement by RIA. Each point represents the average amount of progesterone and testosterone per cell in three independent experiments. Data are representative of two independently conducted experiments.