FIGURE 1.

Domain architecture of DJA2. Top left, proposed arrangement of domains in DJA2 based on Ref. 2. Top right, a homology model of DJA2 was generated by SwissModel using the middle fragment of Ydj1 (1NLT) as template. The N and C termini, m1 and m2 subdomains, zinc-binding sites (Zn-1 and Zn-2), and partial C-terminal (C-term) dimerization region are marked. The positions of the Δm2 deletion and the C-terminal exchange with DJA1 are indicated by dashed lines. Bottom left, diagram of the domain arrangements in the DJA1 and DJA2 primary sequence, and mutants used in this study. The J domain (J), linker (lk), m1 and m2 sequences, zinc-binding sites (Zn-1 and Zn-2), and C-terminal dimerization sites are marked. Bottom right, summary of the results of this study.