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. 2012 Oct 19;287(50):42165–42179. doi: 10.1074/jbc.M112.377374

FIGURE 1.

FIGURE 1.

Isolated liver mitochondria from oral alcohol and intragastric fed mice have a greater respiration than control. A, state III respiration in oral alcohol fed mice. B, state IV respiration in oral alcohol-fed mice. C, RCR in oral alcohol-fed mice. D, state III respiration in intragastric alcohol-fed mice. E, state IV respiration in intragastric alcohol-fed mice. F, RCR in intragastric alcohol-fed mice. Solid bars, control; empty bars, alcohol treatment. State III respiration was measured using either complex I substrates (glutamate/malate, 7.5 mm) or complex II substrate (succinate, 7.5 mm) plus ADP (250 μm) with an oxygen electrode. State IV respiration was measured in the presence of substrates but no ADP. RCR is defined as the state III respiration/state IV respiration ratio. Following 5 weeks of oral alcohol feeding or 4 weeks of intragastric alcohol feeding, liver mitochondria were isolated using differential centrifugation or a Percoll gradient as described under “Experimental Procedures.” n = 5–8 mice/group. Results are mean ± S.D. (error bars); *, p < 0.05 versus control.