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. 2012 Oct 19;287(50):42165–42179. doi: 10.1074/jbc.M112.377374

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effects of alcohol feeding on NAD⁺-NADH levels and redox status in liver homogenate and mitochondria. A, intragastric alcohol model. B, oral alcohol model. Solid bars, control; empty bars, alcohol treatment. Mice were fed alcohol intragastrically or orally, and liver mitochondria were isolated using a Percoll gradient or differential centrifugation. NAD⁺ and NADH levels were measured by HPLC with the fluorescence detector. NAD⁺-NADH levels in liver homogenate are expressed as nmol/mg liver wet weight, whereas mitochondrial NAD⁺-NADH levels are expressed as nmol/mg of protein. n = 4–5 mice/group. Results are mean ± S.D. (error bars); *, p < 0.05 versus control.