FIGURE 10.
Differential regulation of PgDps in response to environmental heme concentrations under iron-depleted conditions. A, relative transcription of dps mRNA normalized against the housekeeping 16S RNA in P. gingivalis cultured under various levels of heme supplementation as the only iron source. Results represent mean value of three independent experiments in triplicate ± S.E. B, PgDps protein production by P. gingivalis grown under different heme supplementation was also quantified by densitometry of Western blot bands. Whole culture samples were normalized to A600 of 1.0 before being separated on SDS-PAGE. Polyclonal anti-Dps antibody was used in the immunoblot assay along with alkaline phosphatase-conjugated secondary antibody. ** denotes p < 0.01.