TABLE 1.
Steady-state kinetic parameters for hpol η-catalyzed formation of mispairs in the presence of WRN
| kcat | Km,dNTP | kcat/Km,dNTP | ƒ (misinsertion frequency)a | |
|---|---|---|---|---|
| min−1 | μm | min−1 μm−1 | ||
| dTTP:dG | 23 ± 1 | 157 ± 25 | 0.15 | 36 |
| WRN1–1092/E84A | 27 ± 4 | 168 ± 54 | 0.16 | 12 |
| dATP:dG | 7 ± 3 | 260 ± 100 | 0.027 | 6.4 |
| WRN1–1092/E84A | 11 ± 2 | 570 ± 200 | 0.019 | 1.4 |
| dGTP:dG | 6 ± 1 | 50 ± 9 | 0.12 | 28 |
| WRN1–1092/E84A | 9 ± 1 | 60 ± 7 | 0.15 | 11 |
a The frequencies of misinsertion were calculated relative to insertion of dCTP opposite dG using the ratio (kcat/Km, mispaired dNTP)/(kcat/Km,dCTP) × 103. The smaller the value of ƒ, the more accurate the polymerase.