FIGURE 4.

Effect of 1,25(OH)₂D₃ supplementation on CSE protein expression and its activity, H₂S formation, GLUT4 total protein expression and its translocation to cell surface, and glucose uptake and glucose utilization in 3T3L1 adipocytes exposed to CG or HG after transfection with CSE siRNA (100 nm). A, CSE protein expression. B, CSE activity. C, H₂S formation. D, GLUT4 total protein expression. E, glucose utilization. F, GLUT4 translocation. G, glucose uptake. Cells transfected with CSE-siRNA were treated with 1,25(OH)₂D₃ for 2 h followed by CG (7 mm) or HG (25 mm) exposure for the next 20 h. Control siRNA is a fluorescein-conjugated scrambled nonspecific RNA duplex with no sequence homology with any of the genes. Values are mean ± S.E. (error bars; n = 3).