FIGURE 5.

Eurocin binding to DPC micelles. A, shown is accumulated chemical shift changes of H^α and H^N atoms of eurocin as calculated by Equation 2 upon the addition of 50 mm DPC. For Pro-30, only the H^α chemical shift change is shown. No data could be obtained from Pro-5 (H^α obscured by H₂O), Trp-31 (cross-peak invisible in spectra at 50 mm DPC), and Pro-36 (H^α vanishes at DPC > 20 mm). B, shown is a fluorescence emission scan (excitation at 295 nm) of eurocin at pH 6 in the absence (dashed line) and presence (solid line) of DPC. a.u., absorbance units. C, shown is a binding isotherm of eurocin to DPC micelles. The dashed line shows the fitted model, whereas circles show individual data points. The inset shows the same data, only zoomed closer to the origin.