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. 2012 Oct 23;287(50):42373–42378. doi: 10.1074/jbc.M112.413674

FIGURE 1.

FIGURE 1.

CCCP induces mitochondrial spheroid formation. A, control or MEFs treated with CCCP for 6 h were fixed and subjected to EM examination. CCCP induced a significant level of ring-shaped mitochondria in MEFs. B, features of ring-shaped mitochondria induced by CCCP included the compression of a portion of the mitochondria to form a quadruple-membrane segment (arrowheads), which seemed to allow the bending of the membrane to circle around cytoplasmic components. These subcellular materials included the cytosol, vesicles (arrows), or a mitochondrion (double arrows). C, wild type MEFs treated with CCCP for 6 h were processed for immuno-EM with anti-Tom20. a, normal mitochondria; b and c, mitochondrial spheroids. The gold particles indicated the Tom20 signals and were present in both the inside and outside of the ring-shaped mitochondria (b and c), suggesting the presence of mitochondrial outer membranes at these locations.