FIGURE 5.

CCCP-induced mitochondrial spheroid formation requires mitofusins. A, wild-type and Mfn1/2^−/− MEFs were preincubated with MitoTracker Red were treated CCCP. Mitochondrial fragmentation was examined by confocal microscopy. B–D, MEFs of different genotypes, with or without the reconstitution of Mfn1 or Mfn2 (D, arrows in the inset; asterisk, nonspecific band), were treated by CCCP for 6 h. Cells were subjected to EM examination, and the percentage of mitochondrial spheroids was quantified.