FIGURE 6.

Loss of mitofusins promotes CCCP-induced mitophagy. A–C, MEFs of different genotypes were infected with ad-GFP-LC3 and treated as indicated. The level of GFP-LC3 puncta (A and B) and LC3-II in cell lysates (C) were determined. D–F, treated MEFs were subjected to EM examination (D). a and b were from wild-type MEFs, whereas c–f were from Mfn1/2^−/− MEFs. An inset in c is enlarged in d. The arrows indicate autophagosomes containing different subcellular components. Asterisks indicated possible mitochondrial remnants (e and f). The number of autophagosomes (E) and the percentage of autophagosomes containing mitochondrial structures (F) were quantified. *, p < 0.05.