FIGURE 3.

Immunofluorescence of the native P10 lens with anti-αA. Different regions of the lens are shown. Central epithelium (CE) is shown in the top panel. Immunofluorescence (red) is seen in the cytoplasm of the lens epithelium without specific definition of immunofluorescence in the apical regions of the epithelium. The difference between immunoperoxidase staining (Fig. 2B) and immunofluorescence shown here may be because of the differential sensitivity of the two techniques, suggesting that there is αA in these cells that is not associated with the apical Golgi. In the proliferative zone (PZ, middle panel); however, the label is predominantly apical (open arrowheads). In the equatorial region (ER, bottom panel), streaks of αA are seen along the elongated nuclei (perinuclear location, thin arrows) in the differentiated fiber cells. The left column shows respective pre-immune controls. Nuclei are stained with DAPI (blue). Scale bar = 100 μm.