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. 2012 Dec 7;3:272. doi: 10.3389/fpls.2012.00272

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Copyright © 2012 Thieme, Schudoma, May and Walther.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Frequency in percent of base-pairing (Watson–Crick or G:U wobble pairs) for each position of the miRNA:miRNA* duplex as predicted by RNAhybrid. The frequency was obtained by dividing the number of sequences in which base-pairing occurred at a given position by the total number of miRNA sequences associated with a particular AGO. “Total” refers to the average of a combined set of all AGO1, AGO2, and AGO5 sequences. Connecting lines are included for visualization purposes only. Note that because the overhanging 3′-end of the mature miRNA sequence, sequence positions up to position 19 were considered only. For those positions, miRNA:miRNA* duplex formation can be assumed as all miRNAs in the set were of length 21 or greater.