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. 2009 Jul;86(1):123–132. doi: 10.1189/jlb.0608345

Figure 3. Comparison of TSG-6 and PTX3 expression in human leukocytes stimulated with pro- and anti-inflammatory stimuli.

Figure 3.

(A) MoDC were differentiated from monocytes and incubated for 4 h with the stimuli indicated; two different concentrations of LPS were used (1 and 10 ng/ml), where this was added to the cultures after 30 min preincubation with IL-10, IL-4 (20 ng/ml), or IFN-γ (5000 U/ml). After total mRNA extraction, TSG-6 and PTX3 expression was determined by Northern blot analysis. The lower panels show the ethidium bromide-stained membranes. The figure shows PTX3 and TSG-6 expression in MoDC in one donor out of the three tested, where these all gave similar results. (B) hPMN were incubated for 4 h or 20 h with LPS (100 ng/ml), alone or in combination with IFN-γ (5000 U/ml), IL-10, IL-4, or IL-13 (20 ng/ml), where the latter was added to cultures 30 min prior to LPS. Ctrl, Control.