Figure 5. Assay of HO-1 Activity Using Mixtures of WT and CYPOR Variants.
Reconstituted systems were prepared with [HO-1] = 0.05 µM and [CYPOR]total = 0.05 µM (open bars) or 0.005 µM (shaded bars), where WT and variant CYPORs were mixed at 1:1. Each system was assayed for HO-1 activity. Each bar represents the mean ± standard deviation of triplicate assays.