. Author manuscript; available in PMC: 2012 Dec 7.

Published in final edited form as: Arch Biochem Biophys. 2011 Jun 28;513(1):42–50. doi: 10.1016/j.abb.2011.06.008

Table 1.

Kinetic parameters extrapolated from curve-fitting of HO-1 activity versus [CYPOR]. Raw data and curve-fitting is shown in Supplemental Figure 2.

CYPOR variant	V_max ^* (nmol/min/nmol)	K_m^CYPOR ^§ (nM)	V_max/K_m (min⁻¹nM⁻¹)	Relative V_max/K_m (% WT)
WT	27.5 ± 1.2	8.6 ± 0.9	3.2 ± 1.3	-
A115V	37.3 ± 7.0	64.5 ± 18.9	0.6 ± 0.4	18
Y181D	ND^#	ND^#	ND^#	-
P228L	33.3 ± 3.0	25.1 ± 4.6	1.3 ± 0.6	42
M263V	37.7 ± 8.7	66.6 ± 23.8	0.6 ± 0.4	18
A287P	18.2 ± 1.2	44.7 ± 7.9	0.4 ± 0.2	13
R457H	26.8 ± 4.5	47.8 ± 13.8	0.6 ± 0.3	18
Y459H	27.8 ± 14.8	1990 ± 1300	0.01 ± 0.01	0
V492E	17.2 ± 3.5	220 ± 110	0.08 ± 0.03	3

Vmax is a mathematical value extrapolated from a Michaelis-Menten plot (Supplemental Fig. 2) and represents the maximal reaction rate were CYPOR not inhibitory of HO-1 activity at higher concentrations.

^§

K_m refers to the [CYPOR] at which half maximal HO-1 activity would be predicted.

ND = not determined because activity below the limit of detection