Table 4. Number of Peptides Unmatched, Mismatched and Correctly Matched at Various Significance Levels by X!Tandem, OMSSA and Crux when Either the b-, y-Ion Series, 50%, or 25% of the Ions in the Query Available for Precursor Charge State +1 and Including Neutral Mass Losses.
OMSSA |
X!Tandem |
Crux |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
significancea | bb | y | 50 | 25 | b | y | 50 | 25 | b | y | 50 | 25 |
Unmtchc | 0 | 0 | 1 | 73 | 79 | 72 | 73 | 295 | 0 | 0 | 0 | 0 |
Mismtchd | 0 | 0 | 0 | 4 | 6 | 15 | 13 | 10 | 0 | 0 | 0 | 0 |
0 | 160 | 48 | 71 | 492 | 237 | 138 | 316 | 1133 | 0 | 2 | 9 | 60 |
1 | 84 | 62 | 72 | 182 | 109 | 113 | 170 | 284 | 93 | 131 | 151 | 322 |
2 | 87 | 86 | 85 | 184 | 149 | 156 | 133 | 228 | 229 | 196 | 243 | 302 |
3 | 100 | 99 | 87 | 178 | 122 | 113 | 166 | 229 | 215 | 155 | 188 | 218 |
4 | 94 | 88 | 106 | 160 | 96 | 98 | 104 | 140 | 154 | 169 | 180 | 183 |
5 | 90 | 89 | 88 | 167 | 105 | 109 | 157 | 136 | 167 | 140 | 188 | 209 |
6 | 64 | 77 | 81 | 133 | 137 | 139 | 109 | 120 | 188 | 173 | 190 | 273 |
7 | 94 | 73 | 86 | 113 | 104 | 105 | 122 | 146 | 167 | 196 | 209 | 326 |
8 | 93 | 90 | 74 | 106 | 89 | 103 | 127 | 131 | 168 | 226 | 244 | 365 |
≥9 | 6984 | 7138 | 7099 | 6058 | 6617 | 6689 | 6360 | 4998 | 6469 | 6462 | 6248 | 5592 |
Prop >6e | 92.2 | 94.0 | 93.5 | 81.7 | 88.5 | 89.6 | 85.6 | 68.7 | 89.1 | 89.9 | 87.8 | 83.5 |
Significance threshold (t) for matched to be considered significant at E- or p-value < 1 × 10–t.
b-, y-ion series, 50%, or 25% of the ions in the query are available.
Unmatched: the program does not provide a match with the program setting.
Mismatched: the program provided an incorrect match.
Percentage of the matches that have E- or p-value < 1 × 10–6.