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. Author manuscript; available in PMC: 2013 Jul 9.
Published in final edited form as: Chembiochem. 2012 Jun 11;13(10):1490–1496. doi: 10.1002/cbic.201200175

Figure 6.

Figure 6

Anti-pTyr dot blot assay. Peptides were normalized to 750 μM, then serially diluted as shown and spotted onto nitrocellulose. The membranes were blocked with bovine serum albumin, probed with anti-pY antibodies 4G10 (above) or PY20 (see Fig. S4), probed with secondary antibody, and then images were developed using chemoluminescent detection. Controls included EGF-stimulated cell lysate (highest concentration is 1 mg/mL total protein), the dye-labeled, pTyr-containing probe peptide used in the fluorescence polarization assays (pTyr-containing ligand), and vehicle (50% DMSO).