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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Chembiochem. 2012 Jul 17;13(12):1818–1825. doi: 10.1002/cbic.201100792

Figure 2. 19 kDa formation is stopped by an m-tyrosinase inhibitor.

Figure 2

A) eDHFR-HA (1.5 mg/ml) was treated with m-tyrosinase (0.15 mg/ml) (left) and (right) experiment was repeated in presence of 1 mM kojic acid. B) Quantitation of Coomassie-stained SDS-PAGE gels showing inhibition of cleavage by kojic acid (1 mM) N=5 (conditions as in A) errors are standard deviations. C) Formation of 19 kDa and loss of 21 kDa band with time, showing lag (conditions as in A). Gel shown in Figure S7.